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SuperArray Bioscience Corporation arraygrade crna cleanup kit
Arraygrade Crna Cleanup Kit, supplied by SuperArray Bioscience Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/arraygrade+crna+cleanup+kit/arraygrade+crna+cleanup+kit/pmc03511871-114-10-14
Average 90 stars, based on 1 article reviews
arraygrade crna cleanup kit - by Bioz Stars, 2026-11
90/100 stars

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Labeling:

Article Title: Soft Tissue Sarcoma Are Highly Sensitive to AKT Blockade: A Role for p53 Independent Up-regulation of GADD45α
Article Snippet: Microarray hybridization Total RNA isolated from STS cells treated with relevant agents was used to synthesize cDNA as template for generating Biotin-16-UTP (non-radioactive) labeled cRNA target using TrueLabeling-AMP linear RNA amplification kit (SuperArray Bioscience Corporation, Frederick, MD). .. Labeled cRNA was purified using SuperArray ArrayGrade cRNA cleanup kit and quantified by spectrophotometry (1 O.D. unit = 40 ug/ml). .. Microarray hybridization Total RNA isolated from STS cells treated with relevant agents was used to synthesize cDNA as template for generating Biotin-16-UTP (non-radioactive) labeled cRNA target using TrueLabeling-AMP linear RNA amplification kit (SuperArray Bioscience Corporation, Frederick, MD).

Article Title: The ATRA-induced differentiation of medulloblastoma cells is enhanced with LOX/COX inhibitors: an analysis of gene expression
Article Snippet: The conversion of the experimental RNA into cDNA and its further transcription and biotin-UTP labeling was performed using TrueLabeling-AMP 2.0 cRNA (SABiosciences, Frederick, MD, USA). .. After purification with the SuperArray ArrayGrade cRNA Cleanup Kit, the labeled target cRNA was hybridized to Human Cancer OHS-802 Oligo GEArray membranes that profile 440 genes (both SABiosciences). ..

Purification:

Article Title: Soft Tissue Sarcoma Are Highly Sensitive to AKT Blockade: A Role for p53 Independent Up-regulation of GADD45α
Article Snippet: Microarray hybridization Total RNA isolated from STS cells treated with relevant agents was used to synthesize cDNA as template for generating Biotin-16-UTP (non-radioactive) labeled cRNA target using TrueLabeling-AMP linear RNA amplification kit (SuperArray Bioscience Corporation, Frederick, MD). .. Labeled cRNA was purified using SuperArray ArrayGrade cRNA cleanup kit and quantified by spectrophotometry (1 O.D. unit = 40 ug/ml). .. Microarray hybridization Total RNA isolated from STS cells treated with relevant agents was used to synthesize cDNA as template for generating Biotin-16-UTP (non-radioactive) labeled cRNA target using TrueLabeling-AMP linear RNA amplification kit (SuperArray Bioscience Corporation, Frederick, MD).

Article Title: Downregulation of gastrokine-1 in gastric cancer tissues and restoration of its expression induced gastric cancer cells to apoptosis
Article Snippet: RNA was then reversely transcribed into cDNA using the TrueLabeling-AMP Linear RNA amplification kit (Superarray, Frederick, MD, USA), and then converted into biotin-labeled cRNA using biotin-16-UTP and an in vitro transcription kit (Roche, Basel, Switzerland). .. The newly synthesized cRNA probes were then purified with the ArrayGrade cRNA cleanup kit (Superarray) and then added to the pretreated Oligo GEArrays Human Apoptosis Microarray (OHS-012 from Superarray) that contains 112 apoptosis-related genes. .. The microarray was then hybridized overnight at 42 o C. The next day, the hybridized arrays were washed and detected by chemiluminescence according to the manufacturer’s instructions (Pierce).

Article Title: Downregulation of gastrokine-1 in gastric cancer tissues and restoration of its expression induced gastric cancer cells to apoptosis.
Article Snippet: RNA was then reversely transcribed into cDNA using the TrueLabeling-AMP Linear RNA amplification kit (Superarray, Frederick, MD, USA), and then converted into biotin-labeled cRNA using biotin-16-UTP and an in vitro transcription kit (Roche, Basel, Switzerland). .. The newly synthesized cRNA probes were then purified with the ArrayGrade cRNA cleanup kit (Superarray) and then added to the pretreated Oligo GEArrays Human Apoptosis Microarray (OHS-012 from Superarray) that contains 112 apoptosis-related genes. ..

Article Title: Nitric Oxide Production Upregulates Wnt/β-Catenin Signaling by Inhibiting Dickkopf-1
Article Snippet: Using the True-Labeling AMP Linear RNA Amplification Kit (SuperArray), the mRNA was reversely transcribed to obtain cDNA and converted into biotin-labeled cRNA using biotin-16-UTP (Roche) by in vitro transcription. .. Before hybridization, the cRNA probes were purified with an ArrayGrade cRNA Cleanup Kit (SuperArray). .. The purified cRNA probes were then hybridized to the pretreated Oligo GEArray Human Wnt Signaling Pathway Arrays (OHS-043, SuperArray), which cover 114Wnt-related genes plus controls.

Article Title: The ATRA-induced differentiation of medulloblastoma cells is enhanced with LOX/COX inhibitors: an analysis of gene expression
Article Snippet: The conversion of the experimental RNA into cDNA and its further transcription and biotin-UTP labeling was performed using TrueLabeling-AMP 2.0 cRNA (SABiosciences, Frederick, MD, USA). .. After purification with the SuperArray ArrayGrade cRNA Cleanup Kit, the labeled target cRNA was hybridized to Human Cancer OHS-802 Oligo GEArray membranes that profile 440 genes (both SABiosciences). ..

Article Title: Mast Cells Are Activated by Streptococcus pneumoniae In Vitro but Dispensable for the Host Defense Against Pneumococcal Central Nervous System Infection In Vivo
Article Snippet: In brief, 3 μg of total RNA that was extracted from frozen brain sections with TRIzol ® reagent (Invitrogen) and pooled from brain extracts of eight mice per group was reverse transcribed. .. Then, biotin-labeled cRNA was synthesized from cDNA with the use of the TrueLabeling-AMPTM Linear RNA Amplification Kit (Superarray). cRNA was purified using the ArrayGrade cRNA Cleanup Kit (Superarray), quantified, and hybridized overnight to inflammatory cytokine and receptor gene-specific probes that were spotted on the GEArray membranes. .. After incubation with streptavidin–AP conjugate, the array image was developed with CPD-Star chemiluminescent substrate (Superarray), recorded with X-ray film, scanned into raw data and analyzed by TINA 2.08e software (Raytest) ( ).

Article Title: Effect of fractionated irradiation on the expression of multidrug resistance genes in the CNE1 human nasopharyngeal carcinoma cell line.
Article Snippet: Using the TrueLabeling-AMP Linear RNA amplification kit (Takara), mRNA was reverse transcribed to obtain cDNA and converted into biotin-labeled cRNA using biotin-16-UTP (Roche Diagnostics, Mannheim, Germany) by in vitro transcription. .. Prior to hybridization, the cRNA probes were purified with the ArrayGrade cRNA cleanup kit (Superarray). .. Purified cRNA probes were hybridized to the pretreated Oligo GEArray Human Cancer Microarrays OHS-044 (Superarray), containing 128 genes associated with 15 signaling pathways.

Spectrophotometry:

Article Title: Soft Tissue Sarcoma Are Highly Sensitive to AKT Blockade: A Role for p53 Independent Up-regulation of GADD45α
Article Snippet: Microarray hybridization Total RNA isolated from STS cells treated with relevant agents was used to synthesize cDNA as template for generating Biotin-16-UTP (non-radioactive) labeled cRNA target using TrueLabeling-AMP linear RNA amplification kit (SuperArray Bioscience Corporation, Frederick, MD). .. Labeled cRNA was purified using SuperArray ArrayGrade cRNA cleanup kit and quantified by spectrophotometry (1 O.D. unit = 40 ug/ml). .. Microarray hybridization Total RNA isolated from STS cells treated with relevant agents was used to synthesize cDNA as template for generating Biotin-16-UTP (non-radioactive) labeled cRNA target using TrueLabeling-AMP linear RNA amplification kit (SuperArray Bioscience Corporation, Frederick, MD).

Synthesized:

Article Title: Downregulation of gastrokine-1 in gastric cancer tissues and restoration of its expression induced gastric cancer cells to apoptosis
Article Snippet: RNA was then reversely transcribed into cDNA using the TrueLabeling-AMP Linear RNA amplification kit (Superarray, Frederick, MD, USA), and then converted into biotin-labeled cRNA using biotin-16-UTP and an in vitro transcription kit (Roche, Basel, Switzerland). .. The newly synthesized cRNA probes were then purified with the ArrayGrade cRNA cleanup kit (Superarray) and then added to the pretreated Oligo GEArrays Human Apoptosis Microarray (OHS-012 from Superarray) that contains 112 apoptosis-related genes. .. The microarray was then hybridized overnight at 42 o C. The next day, the hybridized arrays were washed and detected by chemiluminescence according to the manufacturer’s instructions (Pierce).

Article Title: Downregulation of gastrokine-1 in gastric cancer tissues and restoration of its expression induced gastric cancer cells to apoptosis.
Article Snippet: RNA was then reversely transcribed into cDNA using the TrueLabeling-AMP Linear RNA amplification kit (Superarray, Frederick, MD, USA), and then converted into biotin-labeled cRNA using biotin-16-UTP and an in vitro transcription kit (Roche, Basel, Switzerland). .. The newly synthesized cRNA probes were then purified with the ArrayGrade cRNA cleanup kit (Superarray) and then added to the pretreated Oligo GEArrays Human Apoptosis Microarray (OHS-012 from Superarray) that contains 112 apoptosis-related genes. ..

Article Title: Mast Cells Are Activated by Streptococcus pneumoniae In Vitro but Dispensable for the Host Defense Against Pneumococcal Central Nervous System Infection In Vivo
Article Snippet: In brief, 3 μg of total RNA that was extracted from frozen brain sections with TRIzol ® reagent (Invitrogen) and pooled from brain extracts of eight mice per group was reverse transcribed. .. Then, biotin-labeled cRNA was synthesized from cDNA with the use of the TrueLabeling-AMPTM Linear RNA Amplification Kit (Superarray). cRNA was purified using the ArrayGrade cRNA Cleanup Kit (Superarray), quantified, and hybridized overnight to inflammatory cytokine and receptor gene-specific probes that were spotted on the GEArray membranes. .. After incubation with streptavidin–AP conjugate, the array image was developed with CPD-Star chemiluminescent substrate (Superarray), recorded with X-ray film, scanned into raw data and analyzed by TINA 2.08e software (Raytest) ( ).

Microarray:

Article Title: Downregulation of gastrokine-1 in gastric cancer tissues and restoration of its expression induced gastric cancer cells to apoptosis
Article Snippet: RNA was then reversely transcribed into cDNA using the TrueLabeling-AMP Linear RNA amplification kit (Superarray, Frederick, MD, USA), and then converted into biotin-labeled cRNA using biotin-16-UTP and an in vitro transcription kit (Roche, Basel, Switzerland). .. The newly synthesized cRNA probes were then purified with the ArrayGrade cRNA cleanup kit (Superarray) and then added to the pretreated Oligo GEArrays Human Apoptosis Microarray (OHS-012 from Superarray) that contains 112 apoptosis-related genes. .. The microarray was then hybridized overnight at 42 o C. The next day, the hybridized arrays were washed and detected by chemiluminescence according to the manufacturer’s instructions (Pierce).

Article Title: Downregulation of gastrokine-1 in gastric cancer tissues and restoration of its expression induced gastric cancer cells to apoptosis.
Article Snippet: RNA was then reversely transcribed into cDNA using the TrueLabeling-AMP Linear RNA amplification kit (Superarray, Frederick, MD, USA), and then converted into biotin-labeled cRNA using biotin-16-UTP and an in vitro transcription kit (Roche, Basel, Switzerland). .. The newly synthesized cRNA probes were then purified with the ArrayGrade cRNA cleanup kit (Superarray) and then added to the pretreated Oligo GEArrays Human Apoptosis Microarray (OHS-012 from Superarray) that contains 112 apoptosis-related genes. ..

Hybridization:

Article Title: Nitric Oxide Production Upregulates Wnt/β-Catenin Signaling by Inhibiting Dickkopf-1
Article Snippet: Using the True-Labeling AMP Linear RNA Amplification Kit (SuperArray), the mRNA was reversely transcribed to obtain cDNA and converted into biotin-labeled cRNA using biotin-16-UTP (Roche) by in vitro transcription. .. Before hybridization, the cRNA probes were purified with an ArrayGrade cRNA Cleanup Kit (SuperArray). .. The purified cRNA probes were then hybridized to the pretreated Oligo GEArray Human Wnt Signaling Pathway Arrays (OHS-043, SuperArray), which cover 114Wnt-related genes plus controls.

Article Title: Effect of fractionated irradiation on the expression of multidrug resistance genes in the CNE1 human nasopharyngeal carcinoma cell line.
Article Snippet: Using the TrueLabeling-AMP Linear RNA amplification kit (Takara), mRNA was reverse transcribed to obtain cDNA and converted into biotin-labeled cRNA using biotin-16-UTP (Roche Diagnostics, Mannheim, Germany) by in vitro transcription. .. Prior to hybridization, the cRNA probes were purified with the ArrayGrade cRNA cleanup kit (Superarray). .. Purified cRNA probes were hybridized to the pretreated Oligo GEArray Human Cancer Microarrays OHS-044 (Superarray), containing 128 genes associated with 15 signaling pathways.

RNA Amplification:

Article Title: Mast Cells Are Activated by Streptococcus pneumoniae In Vitro but Dispensable for the Host Defense Against Pneumococcal Central Nervous System Infection In Vivo
Article Snippet: In brief, 3 μg of total RNA that was extracted from frozen brain sections with TRIzol ® reagent (Invitrogen) and pooled from brain extracts of eight mice per group was reverse transcribed. .. Then, biotin-labeled cRNA was synthesized from cDNA with the use of the TrueLabeling-AMPTM Linear RNA Amplification Kit (Superarray). cRNA was purified using the ArrayGrade cRNA Cleanup Kit (Superarray), quantified, and hybridized overnight to inflammatory cytokine and receptor gene-specific probes that were spotted on the GEArray membranes. .. After incubation with streptavidin–AP conjugate, the array image was developed with CPD-Star chemiluminescent substrate (Superarray), recorded with X-ray film, scanned into raw data and analyzed by TINA 2.08e software (Raytest) ( ).

other:

Article Title: Nitric Oxide Production Upregulates Wnt/β-catenin Signaling By Inhibiting Dickkopf-1
Article Snippet: Using the True-Labeling AMP Linear RNA amplification kit (SuperArray), the mRNA was reversely transcribed to obtain cDNA and converted into biotin-labeled cRNA using biotin-16-UTP (Roche) by in vitro transcription.



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